为进一步了解缺氮对油菜养分吸收的影响，以甘蓝型油菜中双１１号为材料，采用营养液培养方法，设置
正常供氮和缺氮两个处理，培养１４ｄ后收获，分析缺氮对油菜植株表型、生物量、植株养分含量及养分吸收相关基
因表达的影响。结果表明，缺氮严重抑制油菜植株生长，地上部生物量显著减少，叶片瘦小、黄化，茎秆呈紫红色。
缺氮促进油菜根系生长，植株根冠比增加。缺氮处理对油菜叶、茎、根中大量元素含量的影响有所不同，缺氮油菜
叶、茎、根的氮浓度及氮含量都显著降低；油菜叶、茎的磷浓度在缺氮胁迫时显著增加，根的磷浓度显著降低，而茎
和根的磷含量在缺氮胁迫时都显著降低，但叶片磷含量不受影响；油菜不同部位钾浓度和钾含量在缺氮胁迫时都
显著降低，并且缺氮处理１４ｄ显著抑制油菜大部分钾通道蛋白基因以及３个根部特异钾转运蛋白基因的表达，说
明缺氮对油菜钾吸收运转相关基因表达的影响，可能导致油菜植株钾含量的降低。

To investigate the effects of N deficiency on nutrients uptake of rapeseed (Brassica napus L.), cultivar Zhongshuang 11 was planted in hydroponics with two N treatments for 14 days. Plant phenotype, biomass, nutrients content and nutrient uptake-related gene expressions were investigated and determined. Result showed that N deficiency extremely repressed rapeseed growth, decreased shoot biomass with thin, chlorotic leaves and purple stems. Nitrogen deficiency promoted root growth with higher root/shoot ratio. The effects of N deficiency on macronutrients uptake were evaluated in different rapeseed tissues. Result showed that N concentration and content in leaf, stem and root were drastically decreased under N deficiency. Phosphorus (P) concentration in leaf and stem were increased by N deficiency, but P content was decreased in root and stem. P content in leaf was not affected by N deficiency. Potassium (K) concentration and content in different tissues were all decreased by N deficiency. In addition, N deficiency notably down-regulated the expressions of K channel genes and 3 root-specific K transporter genes. It suggested that N deficiency significantly influenced the expression of genes related to K uptake and transportation, which might result in decreased K content of rapeseed plants.