利用BSA-Seq方法鉴定向日葵耐盐候选基因

贾秀苹,卯旭辉,岳 云,陈炳东,梁根生,王兴珍

中国油料作物学报 ›› 2018, Vol. 40 ›› Issue (6) : 777.

PDF(3725 KB)
欢迎访问《中国油料作物学报》, 2025年5月2日 星期五
PDF(3725 KB)
中国油料作物学报 ›› 2018, Vol. 40 ›› Issue (6) : 777. DOI: 10.7505/j.issn.1007-9084.2018.06.006
遗传育种

 利用BSA-Seq方法鉴定向日葵耐盐候选基因

  • 贾秀苹(1976-),女,甘肃会宁人,硕士研究生,主要从事向日葵遗传育种研究,E-mail:gsjxp666@163.com #br#
作者信息 +

Identification of major salt-tolerant genes via BSA-Seq method in sunflower

Author information +
文章历史 +

摘要

挖掘向日葵耐盐基因对作物耐盐品种选育具有重要的意义。本研究利用高耐盐自交系Y07-136R(父本)和不耐盐自交系Y05-222A(母本)及其构建的包含600个单株的F2分离群体为试验材料,挑选分离群体中耐盐和不耐盐的极端分离株系各50株单株,分别构建两个DNA池用于高通量测序以分析筛选向日葵耐盐候选基因。通过本研究共发掘到6个耐盐关键候选基因(Ha0_73Ns.730/Ha10.228/Ha12.2377/Ha2.3822/Ha8.182/Ha9.5434),功能注释分别为依赖ATP的RNA解旋酶、热休克蛋白、生长素结合蛋白、溶质运载蛋白家族成员、核糖体蛋白S21家族成员及未知功能蛋白。通过候选基因同源分析、代谢通路分析及共表达基因分析发现,这些基因参与植物逆境胁迫调控,可能具有耐盐的生物学功能。本研究结果为耐盐基因的克隆及功能解析提供了重要候选基因,同时为向日葵耐盐新品种的选育提供了重要的分子标记。


Abstract

Sunflower is known as the pioneer crop in saline soil. Identification of salt-tolerant genes from sunflower has great significance to crop salt-tolerance breeding. In this study, an F2 population with 600 individuals was constructed using salt-tolerant line Y07-136R and salt-sensitive line Y05-222A; 50 F2 salt-tolerant individuals and 50 salt-sensitive individuals were selected respectively to construct 2 DNA pools which were used for high-throughput sequencing. The key candidate genes associated with salt-tolerant genes were identified via BSA-Seq. The results showed that, total 6 key salt-tolerant genes, Ha0_73Ns.730, Ha10.228, Ha12.2377, Ha2.3822, Ha8.182 and Ha9.5434, were identified and the annotation functions were ATP-dependent RNA helicase, heat shock protein, auxin-binding protein, solute carrier family 35 members, putative ribosomal protein S21 family protein and uncharacterized protein. The results of candidate genes homology analysis, metabolic pathway analysis and co-expression analysis showed that these 6 candidate genes may be involved in plant stress regulation. This research provided key salt-tolerant candidate genes and also provides important markers for salt-tolerant breeding in sunflower.

关键词

耐盐基因 / BSA-Seq / 同源分析 / 功能注释 / 向日葵

Key words

 salt-tolerant genes / BSA-Seq / homology analysis / function annotation / sunflower

引用本文

导出引用
贾秀苹,卯旭辉,岳 云,陈炳东,梁根生,王兴珍 .  利用BSA-Seq方法鉴定向日葵耐盐候选基因[J]. 中国油料作物学报, 2018, 40(6): 777 https://doi.org/10.7505/j.issn.1007-9084.2018.06.006
JIA Xiu-ping, MAO Xu-hui, YUE Yun, CHEN Bing-dong, LIANG Gen-sheng, WANG Xing-zhen. Identification of major salt-tolerant genes via BSA-Seq method in sunflower[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2018, 40(6): 777 https://doi.org/10.7505/j.issn.1007-9084.2018.06.006

参考文献

[1]Gupta B, Huang B. Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization[J]. Int J Genomics, 2014(1):701596
[2] Zhu J K, Xiong L. Genetic analysis of salt tolerance in Arabidopsis. Evidence for a critical role of potassium nutrition[J]. Plant
Cell, 1998, 10(7):1181-1191
[3 ] Zhu J K. Salt and drought stress signal transduction in plants[J]. Annu Rev Plant Biol, 2002, 53:247-273
[4] Undurraga S F, Santos M P, Paezvalencia J, et al. Arabidopsis sodium dependent and independent phenotypes triggered by
H+-PPase up-regulation are SOS1 dependent[J]. Plant sci, 2012,183(2):96-105
[5] Liu A L, Zou J, Liu C F, et al. Over-expression of OsHsfA7 enhanced salt and drought tolerance in transgenic rice[J]. BMB Rep, 2013, 46(1):31-36
[6]孙玉莹 ,毕京翠,赵志超,等.作物叶片衰老研究进展[J].作物杂志,2013,4(6):11-19
[7] 蔡晓锋, 胡体旭, 叶 杰,等. 植物盐胁迫抗性的分子机制研究进展[J]. 华中农业大学报, 2015, 34(3):134-141
[8] 唐奇志, 刘兆普, 陈铭达, 等. 海水处理对向日葵幼苗生长及叶片一些生理特性的影响[J]. 植物学报, 2004,21(6):667-672.
[9] 陈全战, 张边江, 周 峰, 等. 钙对盐胁迫条件下油葵幼苗生长速率和抗氧化酶活性的影响[J]. 安徽农业科学, 2008, 36(35):15 336-15 338
[10]Ballesteros E, Blumwald E, Donaire J P, et al. Na+/H+, antiport activity in tonoplast vesicles isolated from sunflower roots induced by NaCl stress[J]. Physiol Plantarum, 1997, 99(2):328-334
[11] M. Ashraf, J. W. O'Leary. Responses of a salt-tolerant and a salt-sensitive line of sunflower to varying sodium/calcium ratios in saline sand culture[J]. J Plant Nutr, 1997, 20(2-3):361-377
[12]郑青松,刘兆普,刘友良,等. 盐和水分胁迫对海蓬子、芦荟、向日葵幼苗生长及其离子吸收分配的效应[J]. 南京农业大学学报, 2004, 27(2):16-20
[13]张俊莲,陈勇胜,武季玲,等. 向日葵对盐逆境伤害的生理反应及耐盐性研究[J]. 中国油料作物学报, 2003, 25(1):45-49
[14]颜 宏,赵 伟,孙玲玲. 水盐浸种对NaCl胁迫下向日葵幼苗渗透调节物质的影响[J]. 作物杂志, 2013(3):131-135
[15]岳 云,李 福,陈炳东,等. 盐胁迫对油葵生理生化指标和产量影响的研究[J]. 作物杂志, 2011 (2):32-35
[16]Almoguera C, Prietodapena P, Díazmartín J, et al. The HaDREB2 transcription factor enhances basal thermotolerance and longevity of seeds through functional interaction with HaHSFA9[J]. BMC Plant Biol, 2009, 9(1):1-12
[17]汤晓丽. 海滨锦葵盐胁迫转录组分析和抗盐基因研究[D]. 中国科学院烟台海岸带研究所, 2016
[18] 张 帆,常李伟,丁明全,等.胡杨果糖-1,6-二磷酸醛羧酶基因PeALD的克隆及耐盐性分析[J]. 基因组学与应用生物学, 2011, 30(3):257-267
[19] 范吉标. 狗牙根耐寒生理及分子机制解析[D]. 中国科学院大学, 2015.
[20] 杨 阳. 基于糜子转录组的NCED基因克隆及干旱胁迫下的表达分[D]. 太谷:山西农业大学, 2015.
[21] Brown J, Pirrung M, McCue LA. FQC Dashboard: integrates FastQC results into a web-based, interactive, and extensible FASTQ quality control tool[J].Bioinformatics. 2017, 33(19): 3137-3139
[22] Bolger A M, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data[J]. Bioinformatics. 2014,30(15):2114-20
[23] Li H, Durbin R: Fast and accurate short read alignment with Burrows-Wheeler transform[J]. Bioinformatics, 2009, 25(14):1754-1760.
[24] Li H, Handsaker B, Wysoker A, et al. The SequenceAlignment/Map format and SAMtools[J]. Bioinformatics, 2009,25(16):2078-2079
[25] McKenna A, Hanna M, Banks E, et al. The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data [J]. Genome Res, 2010, 20(9): 1297-1303.
[26] Wang K, Li M, Hakonarson H. ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data[J]. Nucleic Acids Res, 2010, 38(16):e164.
[27] Obayashi T, Kinoshita K, Nakai K, Shibaoka M, Hayashi S, Saeki M, Shibata D,Saito K, Ohta H. ATTED-II: a database of co-expressed genes and cis elements for identifying co-regulated gene groups in Arabidopsis. Nucleic Acids Res. 2007Jan;35(Database issue):D863-9. Epub 2006 Nov 27. PubMed PMID: 17130150; PubMedCentral PMCID: PMC1716726
[28] Giovannoni J J, Wing R A, Ganal M W, et al. Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations[J]. Nucleic Acids Res, 1991, 19(23): 6553-6568.
[29] Takagi H, Tamiru M, Abe A, et al. MutMap accelerates breeding of a salt-tolerant rice cultivar[J]. Nat Biotechnol, 2015, 33(5):445.
[30 ] Lu H, Lin T, Klein J, et al. QTL-seq identifies an early flowering QTL located near Flowering Locus T in cucumber[J]. theor Appl Genet, 2014, 127(7):1491-1499
[31] Jagadeesan R, Fotheringham A, Ebert P R, et al. Rapid genome wide mapping of phosphine resistance loci by a simple regional averaging analysis in the red flour beetle, Tribolium castaneum[J]. BMC Genomics, 2013, 14(1):1-12.

基金

国家自然科学基金地区基金(31360343);甘肃省特色作物产业技术体系;甘肃省农业科学院科研条件建设及成果转化项目(2016GAAS35);甘肃省生物技术研究与应用开发项目(GNSW-2016-6);特色油料产业技术体系建设项目(CARS-14-2-22)
PDF(3725 KB)

1159

Accesses

0

Citation

Detail

段落导航
相关文章

/