摘要
启动子是基因在转录水平上的一种重要调控元件。本研究通过同源序列法从甘蓝型油菜品种中油821
中分离到油菜基因NapB和FAE1的上游启动子序列pNapB和pFAE1,其中pNapB长1136bp,pFAE1长1420bp。
两种启动子的核苷酸序列都含有种子特异表达启动子的顺式作用元件,包括RY重复、G-box和E-box等,但两
种启动子顺式作用元件的序列分布不同。将pNapB和pFAE1两个启动子分别与报告基因GUS融合并通过农杆菌
介导法导入烟草,得到大量转基因烟草植株。对T1代转基因烟草进行组织化学分析,比较了不同启动子的组织表
达特异性。结果表明,两种启动子都只在种子中起作用,属于典型的种子特异性表达启动子,但是它们作用的时间
和空间分布以及作用强度明显不同。pNapB启动子比pFAE1作用开始的时间早,持续时间长,在早期作用比
pFAE1强,但pFAE1启动子在种子发育中期启动速度快,成熟种子的GUS染色深度在两种启动子间差别不大。
关键词
种子特异表达启动子 /
pNapB /
pFAE1 /
遗传转化 /
GUS /
转基因烟草
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肖 娜,武玉花,肖 玲,吴 刚,卢长明.
油菜种子特异表达启动子pNapB和pFAE1的
结构与功能比较研究
[J]. 中国油料作物学报, 2008, 30(1): 1-9
中图分类号:
S565.403
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肖 娜等:油菜种子特异表达启动子pNapB和pFAE1的结构与功能比较研究9
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脚注
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