植物RNA干扰的研究进展

马建, 刘艺苓, 王丕武

中国油料作物学报 ›› 2008, Vol. 30 ›› Issue (2) : 252-259.

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中国油料作物学报 ›› 2008, Vol. 30 ›› Issue (2) : 252-259.
综述与专论

植物RNA干扰的研究进展

  • 马建,刘艺苓,王丕武*
作者信息 +

The Progress of RNAi in Plant

  • MA Jian,LIU Yi─ling, WANG Pi-Wu*
Author information +
文章历史 +

摘要

RNA干扰(RNA interference。RNAi)是利用双链RNA(dsRNA)特异性地降解相应序列的mRNA,从而特异性地阻断相应基因地表达.在过去10年中,RNA干扰及相关沉默反应的机制研究取得了重大进展, RNAi也发展成为一种强有力的实验工具,用来控制目的基因的表达以获取预期的生物表型。本文详细综述了近年来RNA干扰机制方面的研究进展及RNA干扰在植物中的应用进展。

Abstract

Introduction of double—stranded RNA(dsRNA)into cells can induce specific mRNA degradation,This process is called RNA interference(RNAi).During the past 10 years, tremendous progress has been made in revealing the underlying mechanism of RNAi and related silencing responses. RNAi has become a powerful research tool in regulating the expression of target genes for expected or desirable phenotypes. In this article,the mechanism of RNAi has been described and summarized,and the application of RANi in plant functional genomics has also been reviewed。

关键词

植物 / 转录后基因沉默 / RNA干扰

Key words

Plant / PTGS / RNAi

引用本文

导出引用
马建, 刘艺苓, 王丕武 . 植物RNA干扰的研究进展[J]. 中国油料作物学报, 2008, 30(2): 252-259
MA Jian, LIU Yi─ling, WANG Pi-Wu . The Progress of RNAi in Plant
[J]. CHINESE JOURNAL OF OIL CROP SCIENCES, 2008, 30(2): 252-259

参考文献

[1] Greg Hannon RNAi:A Guide to Gene silencing[M] New York :Cold Spring Harbor Laboratory Press, 2003.5-21
[2]Cameron F. H, Jennings P. A . Inhibition of gene expression with a short sense fragment. [J] Nucleic Acids Res. 1991.,19: 469-474.
[3]Guo S, Kemphues K. J. par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thrkinase that is asymmertrically distributed [J].Cell .1995. 81: 611-620.
[4]Waterhouse P. M, Graham M. W, Wang M. B. Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA[J]. Proc. Natl. Acad. Sci. 1998.95: 13959-13964
[5]Fire A, Xu S, Mello C. C,et a1. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J]. Nature 1998.391: 806-811.
[6]Wassenegger M. RNA-directed DNA methylation[J]. Plant Mol. Biol. 2000.43: 203-220.
[7]Martinez J,Patkaniowska A, Vrlaub H.2002.Single-stranded antisense siRNAs guide target RNA cleavage in RNAi[J]. Cell. 2002.110(5):563-574.
[8]Nykanen A, Haley B, Zamore P. D. ATP requirement and small interfering RNA structure in the RNA interference pathway[J],Cell. 2001.107(3):309-321.
[9]Hutvagner G, Zamore P. D. RNAi: nature abhors a double-stranded[J], Curr. Opin. Genet Dev. 2002.12(2):225-232.
[10] Torgeir Holen, Mohammed Amarzguioui, Eshrat Babaie et a1.2003.Similar behaviour of single-strand and double-strand siRNAs suggests they act through a common RNAi pathway[J].Nucleic Acids Research, 2003, Vol. 31, No. 9 2401-2407
[11] Allison H. S. Hall, Jing Wan, April Spesock, et a1. potency silencing by single-stranded boranophosphate siRNA[J]. Nucleic Acids Research 2006.34(9):2773-2781
[12] Allison H. S. Hall, Jing Wan, et a1.RNA interference using boranophosphate siRNAs: structure–activity relationships[J].Nucleic Acids Research,2004,32(20):5991-6000
[13] Torgeir Holen, Svein Erik Moe, Jan Gunnar et a1. Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo[J]. Nucleic Acids Research . 2005. 33(15):4704-4710
[14]Bender J..A vicious cycle:RNA silencing and DNA methylation in plants[J].CELL 2001.106:129-137
[15]Metle M. F, Aufsatz W, Van der Winden J, et a1. Transcriptional gene silencing and promoter methylation triggered by double stranded RNA[J]. EMBO J. 2000. 19: 5194-5201.
[16]Bartel B, Bartel D P. MicroRNAs:at the root of plant development[J]. Plant Physiol. 2003.132:709-717
[17]Llave C, Xie Z, Kasschau K.D, et a1 Cleavage of scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA[J]. Science. 2002. 297: 2053-2056.
[18]Wianny F, Zernicka-Goetz. M. Specific interference with gene function by double-stranded RNA in early mouse development Nat[J]. Cell Biol. 2000. 2: 70-75.
[19]Cogonz G, Macino G. Gene Silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase[J].Nature. 1999.399:166-169.
[20]Zngelbrecht I, Van Houdt H, Van Montagu M et a1 Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation[J]. Proc. Natl. Acad. Sci.. 1994.91: 10502-10506.
[21]Vaucheret H. Promoter-dependent trans-inactivation in transgenic tobacco plants: kinetic aspects of gene Silencing and gene reactivation[J]. C. R. Acad. Sci. 1992. 317: 310-323.
[22]Vaucheret H. Indentification of a general Silencer of 19S and 35S promoters intransgenic tobacco plants: 90bp of homology in the promoter sequence are sufficient for trans-inactivation[J]. G. R. Acad. Sci. 1993.316: 1471-1483.
[23]Chicas A, Macion G. Characteristics of post-transcriptional gene silencing[J]. EMBO Rep.Z: 2001. 992-996.
[24] Xiaoyu Zhang. Junshi Yazaki.Ambika S et a1 2006 Genome-wide High-Resolution Mapping and Functional Analysis of DNA Methylation in Arabidopsis[J] Cell, 2006 ,126(6):1025-1028
[25]Sijen T, Fleenor J, Simmer F. On the role of RNA amplification in dsRNA-triggered gene silencing[J]. Cell. 2001.107: 465-476.
[26]赵明敏,赵建,郭永清 等. RNA干扰及其在植物研究中的应用 [J].农业生物技术科学2006. 4:36-39
[27] Minsung Kim, Wynnelena Canio, Sharon Kessler, et a1 Developmental Changes Due toLong-Distance Movement of a Homeobox Fusion Transcript in Tomato[J] .Science. 2001. 293 13:287 -289
[28]Voinnet O, Vain P, Angell S, et a1. Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA[J]. Cell,1998. 95: 117-187.
[29]Lucas W. J. Yoo B. C, kragler F. RNA as a long-distance information macromolecule in the plants[J] Nat. Rev. Mol. Cell. Boil. 2001. 11: 849-857.
[30]Berntein E, Caudy A. A, Hamond S. M, et a1 Role for a bidentate ribonuclease in the initiation step of RNA interference[J]. Nature. 2001. 409: 363-366.
[31]Schiebel W, Haas B, Marinkovic S, et a1 RNA –directed RNA polymerase from tomato leaves. I. Purification and physical properties[J]. J. Boil. Chem.. 1993,268: 11851-11857.
[32]Michael w,Gabi K. Nomenclature and functions of RNA-directed RNA polymerases.Trends in Plant[J]. Science. 2006.3:142-152
[33]Ceruttl L, Mian N, Bateman A. Domains in gene silencing and cell differentiation proteins: The novel DAZ domain and redefinition of Piwi domain. Trends Biochem[J]. Sci 2000.25: 481-482.
[34]Carmell M.A, Xuan Z, Zhang. M. Q. The Argonaute family: Tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis[J]. Gene Dev 2002.16: 2733-2742.
[35]Facard M,Boutet S,Morel J.B,et al. AGO1、QDE-2 and RDE-1 are related proteins required for post-transcriptional gene silencing in plants,quelling in fungi,and RNA interference in animas[J].Proc Natl Acad Sic.2000. 97(21):11650-11654
[36] Carmell MA,Xuan Z,ZhaangMQ,et al.The Argonaute family:tentacles that reach into RNAi,developmental control,stem cell maintenance,and tumorigenesis[J].GenesDev,2002,13:2733-2742
[37]Parrish S,Fire A. Distinct roles for RDE-1 and RDE-4 during RNAs interference in Caenorhabditis elegans[J] .RNA .2001.7:1397-1402
[38]Wesley S.V,Helliwell C,A,Smith N.A, ,et al Construct design for efficient effective and high-throughput gene silencing in plants[J].Plant 2001.27:581-590.
[39]FraserA.G,KamathR.S,ZipperlenP, et a1.Functionalgenamic analysis of C.elegans chromosome I by systemic RNA interference[J].Nature. 2000.408:325-330
[40]Schob H,Kunz.C,Meins F.J. Silencing of transgenes introduced into leaves by agroinfiltration :A simple,rapidmethod for investigating sequence requirements for gene silencing[J] .Mol.Gen Genet 1997.256:581-585.
[41]Erik Limpens, Javier Ramos, Carolien Franken, et a1. RNA interference in Agrobacterium rhizogenes-transformed roots of Arabidopsis and Medicago truncatula[J] .Journal of Experimental Botany . 2004.55:983-992
[42]Peele C,Jordan C,V,Muangsan,et al,silencing of a meristematic gene Using gemini virus-derived vectors[J] Plant .2001 ,27:357-366
[43] Zeynep Arziman, Thomas Horn and Michael Boutros . E-RNAi: a web application to design optimized RNAi constructs[J]. Nucleic Acids Research.2005.:W582-W588
[44] Thomas Horn, Zeynep Arziman, Juerg Berger ,et a1. GenomeRNAi: a database for cell-based RNAi phenotypes[J] Nucleic Acids Research.2006.32:492-497
[45] A. Olson, N. Sheth, J. S. Lee, et a1. 2006.RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs[J] Nucleic Acids Research.2006. 34:153-157
[46] Kristin C. Gunsalus*, Wan-Chen Yueh, et a1. RNAiDB and PhenoBlast: web tools for genome-wide phenotypic mapping projects[J] .Nucleic Acids Research. 2004. 32 :406-410
[47] Andreas Henschel, Frank Buchholz , Bianca Habermann . DEQOR: a web-based tool for the design and quality control of siRNAs[J]. Nucleic Acids Research .2004.32(Web Server Issue):W113-W120
[48]Wesley S.V,Helliwell C,A,Smith N.A ,et al Construct design for efficient effective and high-throughput gene silencing in plants[J].Plant.2001. 27:581-590.
[49] Daisuke Miki , Ko Shimamoto .2004. Simple RNAi Vectors for Stable and Transient Suppression of Gene Function in Rice[J] . Plant and Cell Physiology, 2004.45: 490-495
[50]Guo H.S,Fei J.F,Xie Q,et al. A chemical-regulated inducible RNAi system in plants[J].Plant .2003.34:383-392
[51] Zhaosheng.Li, Meina. Y. A novel nuclear-localized CCCH-type zinc finger protein, .OsDOS, is involved in delaying leaf senescence in rice[J]. Plant Physiology.2006..4: 1376-1388
[52]Papon N ,Vansin A ,Gantet P, et al. Histidine-containing phosphotransfer domain extinction by RNA interference turns off a cytokinin signaling circuitry in Cathatanthus roseus suspension cells[J],J FEBS Lett, 2004.558:85~88
[53] Kong, Zhaosheng; Li, Meina. A novel nuclear-localized CCCH-type zinc finger protein, OsDOS, is involved in delaying leaf senescence in rice[J]. Plant Physiology. 2006. 4: 1376-1388
[54] Koga A, Ishibashi T, Kimura S, et al. Characterization of T-DNA insertion mutants and RNAi silenced plants of Arabidopsis thaliana UV-damaged DNA binding protein 2 (AtUV-DDB2) [J].:Plant Molecular Biology. 2006. 4: 227-240
[55] Zhang.Liangran,Tao Jiayi,Wang. Shunxin,et al . The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis. [J] Plant. Mol. Biol. 2006. 4: 533-554
[56]Jensen P E, Haldrup A, Zhang S,et al. The PSI-O subunit of plant photosyststem I is involved in balancing the excitation prossure between two photosystems[J].J Biol Chem.2004.279:24212~24217
[57] Ketelaar T ,Allwood E G, Anothony R,et al. The actin-interacting protein AIP1 is essential for actin organization and plant development[J].J Curr Biol, 2004.14(2):145-149
[58]Stevens R, Grelon M,Vezon D ,et al. ACDC45 homolog in Arabidopsis is essential for meiosis, as shown by RNA interference-induced gene silencing[J].J Plant Cell.2004.16(1):99~113
[59] Daisuke Miki, Rika Itoh and Ko Shimamoto . RNA Silencing of Single and Multiple Members in a Gene Family of Rice [J] .Plant Physiology .2005.138:1903-1913
[60] Senthil Subramanian, Madge Y .Graham, et al. RNA interference of Soybean is oflavone synthase gene leads to silencing in tissues distal to the transformation site and euhanced susceptibility to Phytophthora Sojae[J],Plant Physiology, 2005,137(4):1345-1353
[61]李小平,马媛媛,李鹏丽等,利用RNA干扰敲减rlpk2基因的表达可以延缓大豆叶片衰老[J].科学通报, 2005 ,11(50):1090-1096
[62]Hirotaka Kumagai, Eri Kinoshita, Robert W. et a1.RNAi Knock-Down of ENOD40s Leads to Significant Suppression of Nodule Formation in Lotus japonicus [J].Plant and Cell Physiology ,2006 ,47(8):1102-1111
[63 ]Alice A. Chen, Austin M. Derfus, Salman R. et a1 . Quantum dots to monitor RNAi delivery and improve gene silencing[J] .Nucleic Acids Research . 2005.33(22):e190
[64]Pinto Y.M, Kok R.A,Bandcombe D.C. Resistance to rice yellow mottle virus(RYMV)in cultivated African rice varieties containing RYMV transgenes[J].Nature Biotechnology, 1999.17:702-707
[65]Abbott D,Wang M.B,Waterhouse P.A, A single copy of a virusderived transgene encoding hairpin RNA gives immunity to barley yellow dwarf virus.Molecular[J] Plant Pathology, 2000,1(6):347-356
[66]Tenllado F,Martinez-GarciaB,Vargas M,et al. Crude extracts of bacterially expressed dsRNA can be used to protect plants ngainst virus infections[J].JBMC Biotechonl .2003.3(1):3
[67]Ramanjulu S,Jian-kang Zhu. Novel and stress-regulated microRNAs and other small RNAs form Arabidopsis[J]. The Plant Cell . 2004.16:2001-2019
[68]Fatma K,Guy Charles L. RNA interference of Arabidopsis beta-amylase 8 prevents maltose accumulation upon cold shock and increase sensitivity of PSII photochemical efficiency to freezing stress[J].The Plant Journal.2005.(44):730-734.
[69] Guiliang Tang and Gad Galili. Using RNAi to improve plant nutritional value : from mechanism to application [J]TRENDS in Biotechnology ,2004, 22 (9 ):24-29
[70]李加瑞,赵伟,李全梓等. Waxy基因的RNA沉默使转基因小麦种子中直链淀粉含量下降[J].遗传学报2005.32(8):846-854
[71]Kusaba M,Miyahara K, Iida S, et al. Low glutelin content1: a dominant mutation that suppresses the glutelin multigene family via RNA silencing in rice[J]. Plant Cell. 2003.15:1455-1467
[72]Chioa-Fen Chuang ,Meyerowite E.M.Specific and heritable genetic interference by double stranded RNA in Arabidopsis thaliana[J]. Proc Natl Acad Sci VSA,2000.97:4985-4990
[73]Meilan R ,Branner A M,Skinner J S, et al. Modification of flowering intransgenic trees.[A].Morohoahi N ,komamine A.Moleculatr Breeding of woody plant[C].The Netherland:Elsevier SciBv. 2001,41:247-256
[74]Satoru M, Daisuke M,Masahiro A,et al. RNAi-mediated Silencing of OsGEN-L(OsGEN-like),a new member of the RAD21XPG nuclease family,cause male sterility by defect of microspore development in rice[J].Plant Cell physiol. 2005.46:699-715
[75]M Clure B.A.Haring V,Ebert PR,et al. Style self-incompatibility prouducts of Nicotiana alata are ribonucleases[J].Nature. 1989.342:955-957
[76]O’Brien M,Kapfer C,Major G ,et al. Molecular analysis of the stylar-expressed Solanum chacoense small asparagines-rich protein family related to the HT modifier of gametophy self-incomputibility in Nicotiana [J].Plant 2002. 32(6):985-991

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