中国油料作物学报 ›› 2010, Vol. 32 ›› Issue (2): 320-332 .
• 综述与专论 • 上一篇
唐桂英,柳展基,单 雷*
收稿日期:
1900-01-01
修回日期:
1900-01-01
出版日期:
2010-06-28
发布日期:
2010-06-28
通讯作者:
单 雷
Received:
1900-01-01
Revised:
1900-01-01
Online:
2010-06-28
Published:
2010-06-28
摘要: 二酰基甘油酰基转移酶(DGAT)是生物体内TAG合成过程中的关键酶,它的作用是催化二酰甘油加上脂肪酸酰基形成三酰甘油。本文介绍了DGAT的分类、亚细胞定位、功能、底物特异性和调控,并展望了DGAT研究在油料作物品种改良方面的应用前景。
唐桂英, 柳展基, 单雷. 二酰基甘油酰基转移酶(DGAT)研究进展
[J]. 中国油料作物学报, 2010, 32(2): 320-332 .
[1] Yu Y H, Ginsberg H N. The role of acyl-CoA: diacylglycerol acyIlransferase (DGAT) in energy metabolism[J].Ann Med, 2004, 36 (4): 252-261. [2] Kaup M T, Frose C D, Thompson J E. A Role for Diacylglycerol acyltransferase during leaf senescence[J]. Plant Physiol, 2002, 129: 1 616-1 626. [3] Cases S, Stone S J, Zhou P, et al. Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members[J]. J Biol Chem, 2001, 276(42): 38 870-38 876. [4] Lardizabal K D, Mai J T, Wagner N W, et al. DGAT2 is a new diacylglycerol acyltrans-ferase gene family: purification, cloning, and expression in insect cells of two polypeptides from Mortierella ramanniana with diacylglycerol acyltransferase activity[J]. J Biol Chem, 2001, 276(42): 38 862-38 869. [5] Cases S, Smith S J, Zheng Y W. Identification of a gene encoding an acyl CoA: diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis[J]. Proc Natl Acad Sci USA, 1998, 95(22): 13 018-13 023. [6] Hobbs D H, Lu C, Hills M J. Cloning of a cDNA encoding diacylglycerol acyltransferase from Arabidopsis thaliana and its functional expression[J]. FEBS Lett, 1999, 452: 145-149. [7] Bouvier-Nave P, Benveniste P, Oelkers P, et al. Expression in yeast and tobacco of plant cDNAs encoding acyl CoA: diacylglycerol acyltransferase[J]. Eur J Biochem, 2000, 267: 85-96. [8] He X, Tuoer C, Chen G Q, et al. Cloning and characterizalion of a cDNA encoding diacylg1yccrol acy1transferase from castor bean[J]. Lipids, 2004, 39: 311-318. [9] Nykiforuk C L, Furukawa-Stoffer T L, Huff P, et al. Characterization of cDNAs encoding diacylglycerol acyltransferase from cultures of Brassica napus and sucrose-mediated induction of enzyme biosynthesis[J]. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2002, 1 580(2-3): 95-109. [10] Wang H, Zhang J, Gai J, et al. Cloning and comparative analysis of the gene encoding diacylglycerol acyltransferase from wild type and cultivated soybean[J]. Theor Appl Genet, 2006, 112 (6): 1 086-1 097. [11] Zheng P, Allen W B, Roesler K, et al. A phenylalanine in DGAT is a key determinant of oil content and composition in maize[J]. Nature genetics, 2008, 40 (3): 367-373. [12] Shockey J M, Gidda S K, Chapital D C, et al. Tung (Vernicia fordii) DGAT1 and DGAT2 possess different affinities for eleostearic acid-containing substrates and are localized to different subdomains of the endoplasmic reticulum[J]. Plant Cell, 2006, 18: 2294-2313. [13] Zou J, Wei Y, Jako C, et al. The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene[J]. Plant J, 1999, 19: 645-653. [14] Routaboul J M, Benning C, Bechtold N, et al. The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase[J]. Plant Physiology and Bio-chemistry, 1999, 37(11): 831-840. [15] Sandager L, Gustavsson M H, Stahl U, et al. Storage lipid synthesis is non-essential in Yeast[J]. J Biol Chem, 2002, 277: 6 478-6 482. [16] Neuwald A F. Barth syndrome may be due to an acyltransferase deficiency[J]. Current Biology, 1997, 7:465-466. [17] Kalscheuer R, Steinbüchel A. A novel bifunctional wax ester synthase/acyl-coa: diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1[J]. J Biol Chem, 2003, 278: 8 075-8 082. [18] Saha S, Enugutti B, Rajakumari S, et al. Cytosolic triacylglycerol biosynthetic pathway in oilseeds. Molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase[J]. Plant Physiol, 2006, 141: 1533−1543. [19] Kamisaka Y, Mishra S, Nakahara T. Purification and characterization of diacylglycerol acyltransferase from the lipid body fraction of an oleagious fungus[J]. J Biochem, 1997, 121: 1 107-1 114. [20] Lardizabal K, Effertz R, Levering C, et al. Expression of Umbelopsis ramanniana DGAT2A in seed increases oil in soybean[J]. Plant Physiol, 2008, 148: 89-96. [21] Martin B A, Wilson R F. Properties of Diacylglycerol acyltransferase from spinach leaves[J]. Lipids, 1983, 18: 1-6. [22] Jako C, Kumar A, Wei Y D, et al. Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight[J]. Plant Physiol, 2001, 126: 861-874. [23] Poirier Y, Ventre G, Caldelari D. Increased flow of fatty acid toward beta-oxidation in developing seeds of Arabidopsis deficient in diacylglycerol acyltransferase activity or synthesizing medium-chain-length fatty acids[J]. Plant Physiol, 1999, 121: 1 359-1 366. [24] Xu J, Francis T, Mietkiewska E, et al. Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content[J]. Plant Biotechnol J, 2008, 6(8): 799-818. [25] Lu C, Hills M J. Arabidopsis mutants deficient in diacylglycerol acyltransferase display increased sensitivity to abscisic acid, sugars, and osmotic stress during germination and seedling development[J]. Plant Physiol, 2002, 129: 1 352-1 358. [26] Wilson R F, Kwanyuen P. Triacylglycerol synthesis and metabolism in germinating soybean cotyledons[J]. Biochim Biophys Scta, 1986, 877: 231-237. [27] Lu C L, Noyer S B, Hobbs D H, et al. Expression pattern of diacylglycerol acyltransferase-1, an enzyme involved in triacylgllycerol biosynthesis, in Arabidopsis thaliana[J]. Plant Mol Biol, 2003, 52: 31-41. [28] Sakaki T, Kondo N, Yamada M. Pathway for the synthesis of triacylglycerols from monogalactosyl diacylglycerols in ozone-fumigated spinach leaves[J]. Plant Physiol, 1990, 94: 773-780. [29] Browse J, Somerville C R, Slack C R. Changes in lipid composition during protoplast isolation[J]. Plant Sci, 1988, 56: 15-20. [30] Yen C L E, Monetti M, Burri B J, et al. The triacylglycerol synthesis enzyme DGAT1 also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters[J]. J Lipid Res, 2005, 46: 1 502-1 511. [31] Cao Y Z, Huang A H C. Diacylglycerol acyltransferase in maturing oil seeds of maize and other species[J]. Plant Physiol, 1986, 82: 813-820. [32] Vogel G, Browse J. Choline phosphotransferase and diacylglycerol acyltransferase. Substrate specificities at a key branch point in seed lipid metabolism[J]. Plant Physiol, 1996, 110: 923-931. [33] Löhden I, Frentzen M. Triacylglycerol biosynthesis in developing seeds of Tropaeolum majus L. and Limnanthes douglasii R. Br[J]. Planta, 1992, 188: 215-224. [34] Taylor D C, Weber N, Barton D L, et al. Triacylglycerol bioassembly in microspore-derived embryos of Brassica napus L. cv Reston[J]. Plant Physiol, 1991, 97: 65-79. [35] Ichihara K I, Noda M. Some Properties of diacylglycerol acyltransferase in a particulate fraction from maturing safflower seeds[J]. Phytochemistry, 1982, 21: 1 895-1 901. [36] Ichihara K, Takahashi T, Fujii S. Diacylglycerol acyltransferase in maturing safflower seeds: its influences on the fatty acid composition of triacylglycerol and on the rate of triacylglycerol synthesis[J]. Biochim Biophys Acta, 1988, 958(1): 125-129. [37] Cao Y Z, Huang A H C. Acyl coenzyme a preference of diacylglycerol acyltransferase from the maturing seeds of Cuphea, maize, rapeseeds, and canola[J]. Plant Physiol, 1987, 84: 762-765. [38] Little D, Weselake R, Pomeroy K, et al. Solubilization and characterization of diacylglycerol acyltransferase from microspore- derived cultures of oilseed rape[J]. Biochem J, 1994, 304: 951-958. [39] Sørensen B M, Furukawa-Stoffer T L, Marshall K S, et al. Storage lipid accumulation and acyltransferase action in developing flaxseed[J]. Lipids, 2005, 40: 1 043-1 049. [40] Weselake RJ, Pomeroy MK, Furukawa TL, et al. Developmental profile of diacylglycerol acyltransferase in maturing seeds of oilseed rape and safflower and microspore-derived cultures of oilseed rape[J]. Plant Physiol, 1993, 102: 565-571. [41] Weselake R J, Taylor D C, Pomeroy M K, et al. Properties of diacylglycerol acyltransferase from microspore-derived embryos of Brassica napus[J]. Phytochemistry, 1991, 30: 3 533-3 538. [42] McHenry L, Fritz P J. Cocoa butter biosynthesis: effect of temperature on Theobroma cacao acyltransferases[J]. J Am Oil Chem Soc, 1987, 64: 1 012-1 015. [43] Roughan P G, Slack C R. Cellular organization of glycerolipid metabolism[J]. Annu Rev Plant Physiol, 1982, 33: 97-132. [44] Oo K C, Chew Y H. Diacylglycerol acyltransferase in microsomes and oil bodies of oil palm mesocarp[J]. Plant Cell Physial, 1992, 33: 189-195. [45] Byers S D, Laroche A, Smith K C, et al. Factors enhancing diacylglycerol acyltransferase activity in microsomes from cell-suspension cultures of oilseed rape[J]. Lipids, 1999, 34: 1143−1149. [46] Sanchez J, Cuvillo M T D, Harwood JL. Glycerolipid biosynthesis by microsomal fractions from olive fruits[J]. Phytochemistry, 1992, 31:129-134. [47] Hobbs D H, Hills M J. Expression and characterization of diacylglycerol acyltransferase from Arabidopsis thaliana in insect cell cultrues[J]. Biochem Soc Trans, 2000, 28: 687-689. [48] 王汉中.中国油菜品种改良的中长期发展战略[J].中国油料作物学报,2004, 26(2): 98-101. [49] Bao X, Ohlrogge J. Supply of triacylglycerol in developing embryos[J]. Plant Physiol, 1999, 120: 1 057-1 062. [50] 禹山林,T G Isleib. 美国大花生脂肪酸的遗传分析[J]. 中国油料作物学报, 2000, 22(1): 35-37. |
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