农杆菌介导microRNA398靶基因转化大豆

孙佃臣, ,沙爱华,单志慧,周蓉,陈李淼,周新安

中国油料作物学报 ›› 2011, Vol. 33 ›› Issue (5) : 438-443.

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中国油料作物学报 ›› 2011, Vol. 33 ›› Issue (5) : 438-443.
遗传育种

农杆菌介导microRNA398靶基因转化大豆

  • 孙佃臣1,2 ,沙爱华1,单志慧1,周蓉1,陈李淼1,周新安1
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摘要

拟南芥miR398对低磷胁迫有响应。通过生物信息学方法预测找到大豆miR398a的靶基因铜/锌超氧化物歧化酶GmSODC(Copper/zinc superoxide dismutase),通过RT-PCR扩增得到GmSODC全长cDNA,采用LIC(Ligation-Independent cloning)法将GmSODC连接到双元植物表达载体pJG045上,构建成植物表达载体pSDC6。通过农杆菌介导的子叶节法将GmSODC基因导入大豆品种东农50中,获得了转化GmSODC的T1代植株。采用Real-time PCR的方法,对转基因植株进行GmSODC基因转录水平的相对定量分析,其中1株较非转基因植株表达量明显提高。

关键词

microRNA398 / GmSODC / 大豆 / 遗传转化 / 相对定量

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孙佃臣, ,沙爱华,单志慧,周蓉,陈李淼,周新安 . 农杆菌介导microRNA398靶基因转化大豆
[J]. 中国油料作物学报, 2011, 33(5): 438-443

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