The aim is to compare the differences of seed oil quality among different germplasm of Akebia, and evaluate the quality of different germplasm comprehensively, so as to provide reference for its development and utilization. In this experiment, Akebia plants in the same habitat and growth years were used as materials. The shape and yield traits of 21 Akebia germplasm were measured and recorded. The oil content was determined by Soxhlet extraction method, and fatty acid types and contents were determined by gas chromatography. The yield and quality differences between species and within species were analyzed by coefficient of variation. Through correlation analysis, principal component analysis and grey correlation analysis, comprehensive analysis and evaluation were also carried out. Results showed that oil content, acid value, peroxide value, iodine value and saponification value of 21 accessions of Akebia were 33.86-47.08%, 2.64-11.72 KOHmg·g-1, 0.03-0.38 g·100g-1, 47.33-81.85 g·100g-1 and 237.87-281.53 KOHmg·g-1, respectively. The relative mass fraction of saturated fatty acids ranged from 13.56% to 18.51%. Oleic acid and linoleic acid were the main unsaturated fatty acids, and their relative mass fraction ranged from 45.75% to 66.17%. Differences were found among interspecific and intraspecific. The yield traits of Akebia trifoliate (Thunb) Koidz was significantly different, and that of Akebia trifoliatr (Thunb) Koidz.var. australis (Diels) Rehd was larger in oil quality. Correlation analysis showed that the fruit quality and shape index were correlated with some quality indexes of seed oil (single fruit quality was significantly negatively correlated with saturated fatty acids; fruit shape index was positively correlated with oil content, oleic acid, monounsaturated fatty acid, saponification value, and negatively correlated with peroxide value), and the higher the two, the higher the quality of seed oil. Principal component analysis and grey weighted correlation analysis showed that the best germplasm was Akebia trifoliatr (Thunb) Koidz.var. australis (Diels) Rehd (B1-B8). At the same time, S6 and S8 of Akebia trifoliate (Thunb) Koidz ranked above the middle in the two kinds of analysis, which was worthy of development and utilization. Fruit color and shape had little effect on the comprehensive quality of seed oil. The comprehensive quality of seed oil from B1 to B8, S6 and S8 was better, thus has higher utilization value.