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中国油料作物学报 ›› 2021, Vol. 43 ›› Issue (2): 314-.doi: 10.19802/j.issn.1007-9084.2019307

• 土肥植保 • 上一篇    下一篇

四川带状套作大豆种腐病菌的分离与鉴定

常小丽(1983-),女,副教授,山西曲沃人,博士后,主要从事作物真菌病害研究,E-mail: xl_changkit@126.com   

  1. 四川农业大学农学院/四川省作物带状复合种植工程技术中心,四川成都,611130
  • 出版日期:2021-04-28 发布日期:2021-04-14
  • 通讯作者: 杨文钰(1958-),男,教授,主要从事间套作栽培理论及技术研究,E-mail: mssiyangwy@sicau.edu.cn
  • 基金资助:
    国家重点研发计划项目(2018YFD0201006);国家自然科学基金(31801685);大豆产业技术体系(CARS-04-PS19)

Isolation and identification of the fungi causing soybean seed decay in maize-soybean relay strip intercropping in Sichuan

  1. College of Agronomy, Sichuan Agricultural University / Sichuan Engineer Research Center for Crop Intercropping
    System, Chengdu 611130, China
  • Online:2021-04-28 Published:2021-04-14

摘要: 明确套作大豆种腐病菌的种类及其致病性,对于防治大豆种腐病,提高大豆产量和品质具有重要意义。
于2016~2018年从四川仁寿、崇州、南充等地收集大豆种腐病的病籽粒,通过组织分离与纯化,基于形态特征、rDNA
ITS和EF-1α 序列分析以及致病性测定,鉴定四川套作大豆种腐病的病原菌种类。结果表明:分离获得的70株菌株
被鉴定为藤仓镰孢菌Fusarium fujikuroi、木贼镰孢菌F. equiseti、亚洲镰孢菌F. asiaticum、大豆茎溃疡病菌Diaporthe
phaseolorum 和大豆北方茎溃疡病菌D. phaseolorum var. caulivora,分离频率分别为62.86%、21.43%、10%、4.29% 和
1.43%。致病性检测表明,各代表菌株均能够侵染套作大豆南豆12,引起种子腐烂,发芽率降低,芽长变短,且以F.
asiaticum 和D. phaseolorum 接种后菌丝覆盖面积、发病率和病情指数最高。本研究初步明确了镰孢菌属Fusarium
spp.和间座壳属Diaporthe spp.是四川套作大豆种腐病的致病菌,为大豆抗种腐病品种选育及病害防治提供了依据。

关键词: 玉米-大豆带状套作, 大豆种腐病, 镰孢菌, 致病性

Abstract: For effective management of soybean seed decay and improvement of soybean yield and quality, it is
significantly important to find out the fungal species and their pathogenicity of soybean seed decay in maize-soy⁃
bean relay strip intercropping. During 2017-2018, the infected soybean seeds were collected from Renshou, Chong⁃
zhou, Nanchong of Sichuan Province, China, respectively. The fungi were isolated from these infected seeds and pu⁃
rified by hypha tip method, and identified based on morphological characteristic and sequence analysis of rDNA ITS
and EF-1α genes. The pathogenicity tests of the representative isolates were further conducted. Our results showed
that a total of 70 isolates were identified as 5 fungal species including Fusarium fujikuroi, F. equiseti, F. asiaticum,
Diaporthe phaseolorum and D. phaseolorum var. caulivora, with the separation frequency of 62.56 % , 21.43%,
10.00 %, 4.29 % and 1.43 %, respectively. Furthermore, the pathogenicity tests showed that all of the representa⁃
tive isolates belonging to five specie were able to cause seed decay of the intercropped soybean cultivar ND12 and
reduced seed germination and germ length. Fusarium asiaticum and D. phaseolorum had the highest mycelium cov⁃
erage area, and disease incidence with the disease index of 100. This study firstly reports that the Fusarium spp.
and Diaporthe spp. are the dominant pathogens of soybean seed decay in the maize-soybean relay strip intercrop⁃
ping system, which will provide basis for breeding of soybean resistant cultivar and disease management.

Key words: maize-soybean relay strip intercropping, soybean seed decay, Fusarium spp., pathogenicity

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