[ 1 ] Murphy D J , Cummins I, KangA S. Synthesis of thema2
jor oil - body membrane p rotein in develop ing rapeseed
(B rassica napus) embryos. Integration with storage - lip2
id and storage - p rotein synthesis and imp lications for the
mechanism of oil - body formation[ J ]. Biochem J , 1989,
258 (1) : 285—93.
[ 2 ] GramiB, Baker R J , Stefansson B R. Genetics of p rotein
and oil content in summer rape. Heritability, number of
effective factors and correlations [ J ]. Can J Plant Sci,
1977, 57: 937—943.
[ 3 ] Sugimoto T, Tanaka K, Monma M, et al. Phospho -
enolpyruvate carboxylase level in soybean seed highly
correlates to its contents of p rotein and lip id [ J ]. Agri
Bio Chem, 1989, 53 (3) : 885—887.
[ 4 ] 陈锦清,黄锐之,郎春秀,等. 油菜PEP基因的克隆及
PEP反义基因的构建[ J ]. 浙江大学学报(农业与生命
科学版) , 1999, 25 (4) : 365—367.
[ 5 ] 陈锦清,郎春秀,胡张华,等. 反义PEP基因调控油菜
籽蛋白质/油脂含量比率的研究[ J ]. 农业生物技术学
报, 1999, 7 (4) : 316—320.
[ 6 ] 张银波,江木兰,胡小加. 油菜PEPase基因的克隆及
其对应RNAi载体的构建[ J ]. 中国油料作物学报,
2005, 27 (1) : 1—4.
[ 7 ] 汪承刚,张菊红,谢 妤,等. 甘蓝型油菜依赖焦磷酸
的磷酸果糖激酶PFK基因片段克隆及hpRNAi载体构
建[ J ]. 分子植物育种, 2006, 4 (2) : 199—204.
[ 8 ] 李 佳,沈斌章,韩继祥,等. 一种有效提取油菜叶片
总DNA的方法[ J ]. 华中农业大学学报, 1994, 13:
521—523.
[ 9 ] Ryan A J , Royal C L, Hutchinson J , et al. Genomic se2
quence of a 12S seed storage p rotein from oilseed rape
(B rassica napus c. v. jet neuf) [ J ]. Nucleic Acids Res,
1989, 17 (9) : 3584.
[ 10 ] Josefsson LG, LenmanM, EricsonM L, et al. Structure
of a gene encoding the 1. 7 S storage p rotein, nap in,
from B rassica napus[ J ]. J Biol Chem, 1987, 262 (25) :
12196—12201.
[ 11 ] Yanai Y, Okumura S, Shimada H. Structure of B rassica
napus phosphoenolpyruvate carboxylase genes: missing
introns causing polymorphisms among gene family mem2
bers. Biosci. Biotechnol [ J ]. Biochem, 1994, 58 ( 5) :
950—953.
[ 12 ] Kohno - Murase J , Murase M, Ichikawa H, et al.
Effects of an antisense nap in gene on seed storage com2
pounds in transgenic B rassica napus seeds [ J ]. Plant
Molecular Biology, 1994, 26: 1115—1124.
[ 13 ] Waterhouse PM, Smith N A, WangM B. Virus resist2
ance and gene silencing: killing the messenger [ J ].
Trends Plant Sci, 1999, 4: 452—457.
[ 14 ] Waterhouse PM, WangM B, Lough T. Gene silencing
as an adap tive defense against viruses [ J ]. Nature,
2001, 411: 834—842.
[ 15 ] Matzke M A, Matzke A J , Pruss G J , et al. RNA -
based silencing strategies in p lants[ J ]. CurrOp in Gen2
etDev, 2001, 2: 221—227.
[ 16 ] Vance V, Vaucheret H. RNA silencing in p lants: de2
fense and counterdefense [ J ]. Science, 2001, 292:
2277—2280.
[ 17 ] Wesley S V, Helliwell C A, Smith N A. Construct de2
sign for efficient, effective and high - throughput gene
silencing in p lants [ J ]. The Plant J , 2001, 27 ( 6 ) :
581—590.
[ 18 ] 肖 玲,卢长明. 以油菜脂肪酸延长酶基因fae1为靶
标RNAi载体的构建[ J ]. 中国油料作物学报, 2004,
26 (4) : 6—11.
[ 19 ] Stoutjesdijk P A, Singh S P, L iu Q, et al. hpRNA -
mediated targeting of the A rabidiosis FAD2 gene gives
highly efficient and stable silencing[ J ]. Plant Physiol,
2002, 129 (4) : 1723—1731.
汪承刚等:甘蓝型油菜三种种子储藏蛋白和丙酮酸羧化酶基因片段的克隆及hpRNA表达载体的构建249
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