The biosynthesis of gibberellin（GA）is a process of various enzymatic reactions and is closely related to plant development and stress response. To explore the distribution, structure and activity of sesame GA related genes, sesame genome sequence and transcriptomes from different tissues were studied based on bioinformatics methods. It showed that sesame had 32 GA related genes belonging to 7 different gene families, and these genes were distributed on all chromosomes except the chromosome 5. The protein sequence lengths of the different gene families were quite different. The CPS genes had the longest protein and contained 776 amino acid residues on average. The KAO proteins exhibited a great variation in size, varying from 401 to 834 amino acids. All the proteins encoded by GA related genes were predicted to be hydrophilic proteins. Compared with other species inclusing Arabidopsis, Rice, Soybean, Grape, Sorghum and Tomato, the total number of GA related genes in sesame is not outstanding, but more CPS genes were observed in sesame. Evolution analysis indicated that sesame and tomato had a closer evolution relationship among the seven species. In various sesame tissues, the 7 GA related gene families displayed quite different expression patterns. KS genes were highly expressed in seeds, stem tips and leaves. CPS genes were more active in pericarps and seeds, but were hardly expressed in stem tips and leaves. KAO and KO genes were constitutively and higly expressed in all tissues, while KS and GA3ox genes showed low levels of expression. Among the different tissues, the GA related genes generally showed higher activity in the seed, then in the root tip and in the stem tip. This study provided important genetic information and theoretical references to analyze the characteristics of GA related genes in sesame and its role in different tissues.